Practcal uses sperm



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Since ICSI circumvents all natural barriers to fertilization, the success rate of the technique is dependent on the quality of the spermatozoa selected for injection [ 58 ]. The process takes place in the female genital tract but can be induced in vitro by incubating spermatozoa with capacitation-inducing media. The interaction between spermatozoa and the zona pellucida is a critical event leading to fertilization and reflects multiple sperm functions ie, completion of capacitation as manifested by the ability to bind to the zona pellucida and to undergo ligand-induced AR. Normally, semen coagulates upon ejaculation and usually liquefies within min. Sperm FISH analysis may be useful in the following: The only thing that matters is the DNA inside the sperm. Comparison of three methods to detect white blood cells in semen:
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Therefore, it is not certain that it would be possible to sex spermatozoa in sufficient numbers with colloid centrifugation to be useful for AI doses. Thus the composition of the cryopreservation medium is important for supporting capacitation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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